Appraisal of the Treponema pallidum haemagglutination test.
نویسندگان
چکیده
Several attempts have been made to use haemagglutination reactions for the diagnosis of syphilis between 1932 and 1958. The first reliable method was developed by Rathlev (1965). Further results of investigations with this method have been reported by authors from Japan and from the Venereal Disease Research Laboratory in Atlanta, U.S.A. Rathlev prepared the reagents for the test in the Statens Seruminstitut, Copenhagen, Denmark, but the other investigators used reagents obtained from Fujizoki, Tokyo, Japan. This paper presents the first results of the TPHA test obtained in the Serodiagnosis Laboratory at the Department of Dermatology in the Vienna City-Lainz Hospital.
منابع مشابه
Treponema pallidum haemagglutination assay in the routine serodiagnosis of treponemal disease.
A passive haemagglutination test using sheep erythrocytes sensitised with Treponema pallidum extract was introduced into syphilis serology by Rathlev (1965, 1967). Shortly afterwards, Tomizawa and Kasamatsu (1966) and Tomizawa, Kasamatsu, and Yamaya (1969) described a similar test, the Treponema pallidum haemagglutination assay (TPHA), and this was further developed as an automated quantitative...
متن کاملTreponema pallidum specific IgM haemagglutination test for serodiagnosis of syphilis.
The Treponema pallidum specific IgM haemagglutination (TP-IgM-HA) test uses erythrocytes sensitised with antiserum to human IgM to separate IgM from IgG in serum. Specific antitreponemal IgM captured in this way is detected by adding a second reagent comprising erythrocytes sensitised with T pallidum antigen. Eighty two serum samples from 82 patients with untreated syphilis, 521 samples from 73...
متن کاملReiter haemagglutination test: a screening test for syphilis.
Using an ultrasonicate of the Reiter treponeme as antigen the Reiter haemagglutination test (RHA) was evaluated as a serological test for syphilis. Comparison of the results of the cardiolipin Wassermann reaction, Reiter protein complement-fixation test, the fluorescent treponemal antibody-absorbed (FTA-ABS) test, the Treponema pallidum haemagglutination test (TPHA) (at dilutions of 1/16 and 1/...
متن کاملThe Treponema pallidum haemagglutination (TPHA) test in biological false positive and leprosy sera.
The Treponema pallidum haemagglutination (TPHA) test was carried out on 274 sera known to show biological false positive reactions to reagin tests for syphilis. The Treponema pallidum immunization (TPI) and fluorescent treponemal antibody absorption (FTA-ABS) tests were non-reactive on all these sera. Thirty-one or 11.3% showed reactive results in the TPHA test. Sera from 267 people who had lep...
متن کاملTreponema pallidum haemagglutination test as a screening procedure for the diagnosis of syphilis.
The serological diagnosis of syphilis relies upon the widespread use of non-specific screenig tests, such as the cardiolipin Wassermann reaction (CWR) and the Venereal Diseases Research Laboratory (VDRL) slide test, in combination with the Reiter protein complement-fixation (RPCF) test (British Cooperative Clinical Group, 1972). Where necessary, the fluorescent treponemal antibody-absorbed (FTA...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The British journal of venereal diseases
دوره 49 2 شماره
صفحات -
تاریخ انتشار 1973